Pulse™: Cell line development from design to GMP master cell bank

Pulse™ is a pre-established, iPSC-derived Cell Line Development platform that streamlines the generation of a GMP Master Cell Bank, providing a consistent, scalable foundation for the entire lifecycle of your cell therapy product. 

 Yes. Cellistic provides both unedited iPSC lines and gene-edited Allo Chassis™ iPSC lines. We can also generate customer-specific cell lines tailored to your needs. 

 Yes, we’re flexible. We can generate iPSC lines from your donor material to generate your GMP MCB. 

 We use automated, closed-system platforms to isolate single iPSCs and expand them into clonal lines. This allows us to select the best-performing clone while ensuring each line is monoclonal, consistent, and reproducible for therapeutic use.

We maintain low passage numbers, use standardized culture conditions, and have rigorous quality controls to ensure iPSC lines remain genetically stable.

Cellistic works exclusively with iPSCs. If you’re considering switching from autologous or donor-derived allogeneic material, we can provide proof-of-concept support to help you explore the transition. 

By combining STAR-CRISPR multiplex gene editing with automation, Pulse™  can reduce your cell line development timeline by 12 months or more compared to traditional approaches. 

Yes. Cellistic’s facilities are EMA-inspected and certified, and operate in compliance with FDA and PMDA standards.

Yes. We provide comprehensive GMP documentation, including Certificates of Analysis, batch records, and traceability logs. 

Our platform offers different entry points and combines proven, validated workflows with flexible steps that can be tailored to your program’s specific needs.

Yes. A license is required for STAR-CRISPR™. For Allo Chassis™, a license is needed if you use our gene-edited cell lines as starting material.

Yes. While Cellistic’s proprietary STAR-CRISPR™ technology is fully owned and integrated into our platform, our team also has experience with other gene-editing technologies and can support their use in your program.

 No third-party licenses are required. 

Yes, we have in-house and outsourced analytical methods.

Yes, you can tech transfer your own analytical methods. We will work with you to evaluate and implement this as a customized option. 

STAR-CRISPR™ combines optimized guide design with high-fidelity nucleases, achieving higher editing efficiency and lower off-target effects than other technologies. All edits are rigorously validated to confirm on-target precision, specificity, and reproducibility.